WebIn most polar solvents, Nile red will not fluoresce; however, when in a lipid-rich environment, it can be intensely fluorescent, with varying colors from deep red (for polar membrane lipid) to strong yellow-gold emission (for neutral lipid in intracellular storages). WebApr 21, 2024 · To overcome these limitations, we developed three novel extracellular vesicular-membrane-binding fluorescent probes-Mem dye-Green, Mem dye-Red, and Mem dye-Deep Red-for monitoring EV uptake into cells. The dyes contain a cyanine group as a fluorescent scaffold and amphiphilic moieties on the cyanine.
Mannose metabolism inhibition sensitizes acute myeloid …
WebPhospholipid with DPH-Labeled Acyl Chain. Phospholipids with NBD-Labeled Acyl Chains. Phospholipids with Pyrene-Labeled Acyl Chains. Phospholipids with a Fluorescent or Biotinylated Head Group. LipidTOX Phospholipid and Neutral Lipid Stains for High … WebAug 17, 2016 · Detailed protocols for some of the most popular and efficient contrasting methods are provided. Lipid Droplets (LDs), also known as lipid bodies, oil bodies or … onst41pb
Looking for a lipid stain that does not interact with protein for ...
WebFeb 1, 2024 · Indeed, the last decade has enriched the toolbox of general lipid stains, fluorescent lipid analogues, sugar-binding lectins, and protein-specific antibodies enabling the first rationally designed plasma membrane-specific dyes. Still, multiple challenges exist, and the unique properties of each dye must be considered when selecting a staining ... WebApr 7, 2024 · After staining with ROS-specific fluorescent dihydroethidium [DHE; dissolved in dimethyl sulfoxide (DMSO) to 2 mg / mL and diluted with PBS at a ratio of 1:500] dye (D7008; Sigma) at 37°C for 30 min and three rounds of PBS washes on a decolorizing shaker (5 min each), samples were incubated with 4′,6-diamidino-2-phenylindole (DAPI; … WebLipids and Lipid Derivatives. ... BODIPY TR ceramide is a red-fluorescent dye that can be used to stain Golgi apparatus in live cells. For Research Use Only. Not for use in diagnostic procedures. Specifications. Recommended Storage. Store in freezer (-5 to -30°C) and protect from light. ons table rpi