Optical fan for single-cell screening
WebJan 19, 2024 · An optical approach for cell enrichment by patterned illumination followed by FACS sorting We developed an approach, which we term optical enrichment, to select cells of interest using a microscope and mark them by photoactivation, enabling cell isolation using FACS ( Fig. 1 ). WebJan 2, 2024 · An optical fan was demonstrated to screen leukemia cells from the blood sample at the single-cell level in a noninvasive and noncontact manner. Further details can be found in the article by Xiaoshuai Liu, Yuchao Li, …
Optical fan for single-cell screening
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WebJul 19, 2024 · The single-cell screening has attracted great attentions in advanced biomedicine and tissue biology, especially for the early disease diagnosis and treatment monitoring. In this work, by using a specific-designed fiber probe with a flat facet, we propose an “optical fan” strategy to screen K562 cells at the single-cell level from a ... WebCorrelation of genetic information with its single cell of origin requires an approach to isolate single cells from a tissue. Recently, approaches have been used for the selection and transfer of single cells into wells including laser capture microdissection, optical tweezer manipulation, flow cytometry (FCM), and microfluidic methods.
WebDec 23, 2024 · December 23, 2024 Optical 'tweezer' enables fast, low-cost screening of bacteria and cancer cells by Li Yuan, Chinese Academy of Sciences Versatile, facile and low-cost single-cell... WebOptical tweezers provide a powerful tool to trap and manipulate living cells, which is expected to help people gain physiological insights at single-cell level. However, trapping and manipulating single cells under crowded environments, such as blood vessels and lymph nodes, is still a challenging task. To overcome this issue, an annular beam formed …
WebAug 1, 2024 · Optical fan for single-cell screening. J. Biophot., 13 (2024), Article e202400155, 10.1002/jbio.202400155. View in Scopus Google Scholar [22] Y. Xie, H. Bachman, T.J. Huang. Highly efficient single cell arraying by integrating acoustophoretic cell pre-concentration and dielectrophoretic cell trapping. WebAug 5, 2024 · This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.
Webfluidic chip, an optical time-stretch quantitative phase microscope, and a digital image processor equipped with machine learning. As a result, it provides both the opacity and phase maps of every single cell at a high throughput of 10,000 cells/s, enabling accurate cell classification without the need for fluorescent labeling.
WebOptical pooled screening in a 2D or 3D co-culture system could be used to analyze non-cell autonomous phenotypes based on physical contact (e.g., formation of adhesion complexes, direct contact signaling, neurotransmission). Existing protocols for in situ sequencing in tissue samples (. Ke et al., 2013. norm beuthWebThe single-cell screening platform was successfully fabricated by replicating each PDMS layer (the top control layer, the middle control layer, and the bottom cell culture/analysis layer) from the mater molds and assembling all the layers together (SI Fig. S1A-C). norm berthWebAug 13, 2024 · MR: One of the major challenges in single-cell analysis is the initial isolation and culture of a single cell. Cell viability is required for downstream analysis and further cell outgrowth. However, cells are not usually on their own and can be difficult to culture independently. Cells are heavily influenced by their surrounding environment and ... normbrecherWebThe single-cell screening has attracted great attentions in advanced biomedicine and tissue biology, especially for the early disease diagnosis and treatment monitoring. In this work, by using a specific-designed fiber probe with a flat facet, we propose an "optical fan" strategy to screen K562 cells at the single-cell level from a populations of RBCs. After the 980-nm … norm boardmanWebThe single‐cell screening has attracted great attentions in advanced biomedicine and tissue biology, especially for the early disease diagnosis and treatment monitoring. In this work, by using a specific‐designed fiber probe with a flat facet, we propose an “optical fan” strategy to screen K562 cells at the single‐cell level from a ... norm black duvall waWebFeb 10, 2024 · The use of sophisticated models such as organoids and whole organisms, flexible perturbations such as gene activation and repression, diverse biological challenges and data-rich read-outs such as... norm blechWebFeb 27, 2024 · Free eye exams for infants. InfantSEE is a national public health program managed by the American Optometric Association (AOA). The program is funded through AOA's charitable organization, Optometry Cares - The AOA Foundation. The InfantSEE program provides a one-time, free eye exam for infants. how to remove virus from cell phone